Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax Multi-Mode Microplate Readers. 5 ng/ml; estradiol: 10 pg/ml. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). See More Microplate Reader / Microtiter Plate Reader. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Molecular Devices Spectramax 340PC instructions. , Sunnyvale, Calif. 2. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). SpectraMAX 340. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Optical density was read at 405 nm after 30 min using a Molecular Devices SpectraMax 340 spectrophotometer. SpectraMax Multi-Mode Microplate Readers. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Statistical analysis. Details. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. All assays were performed using a sample size of 12, and the absorbance at 425 nm was measured by using a SpectraMax 340 multiwell plate reader (Molecular Devices, Sunnyvale, CA). The values were corrected for nonspecific background by subtracting the OD 650. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. Cell permeabilization. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. The ratio of red to green fluorescence intensity was calculated. The initial rate of hydrolysis was determined using the early linear region of. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. ). Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. Calculating % of total mediator release in RBL assay. 8. To measure the cell biomass (g/L),. To. Stat Fax 4700 Microstrip Reader. All strains were tested three times and the results averaged. SpectraMax Plus 384 measuring instruments pdf manual download. 5. Call 1-800-745-2710. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 570nm on the indicated days (Cell Proliferation Kit I; Roche). Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 1% PEG 8000 at 37°C. Inter-assay variation was prevented by testing all of the samples on the same day. Temperature Range: Ambient + 4 °C up to 45 °C. PerkinElmer. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). Acceptor: 665nm. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. 7 macrophages were obtained from ATCC (Manassas, VA). This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. 5mM), and changes in OD 405 nm. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 1% N-1-naphtylethylenediamine dihydrochloride, 2. plates can be used in the SPECTRAmax PLUS 384. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. . Glutathione reductase (GR) activity was measured, according to Smith et al. well, and strip-well microplates. One plate carrier adapter is provided with the instrument. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). , Sunnyvale CA). Revvity. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. 0% and ± 0. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. All data were normalized to experimental day 0. Spectrophotometer (SpectraMax 340). ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. Figure 1. , Sunnyvale, CA, USA) at a wavelength of 450 nm. 1 mL of culture media with 10% FBS. ). The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). Data are expressed as percent of maximum binding for each protein. The loading equaled the mass of BSA per mass of particles. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Both readers feature an 8-channel read head for fast reads and. 0. Introduction. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. 5. The plate was covered and incubated for one hour at room temperature. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Driessen*,MaaikeA. The slope of the linear portion of the kinetic curve generated from each well was used to. 3. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. Tunable for filterless flexibility. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). 9000-0756. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Lactate Dehydrogenase (LDH) Release. Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. High fg levels of Streptavidin High fg levels of Streptavidin Exceeds Cisbio certification requirements TRF-based with 340/80 nm EX and 616/10 nm EM AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm. Figure 1. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 340 nm / 80 nm. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Oxidative stress induction by H 2 O. StakMax. 6) and 0. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). reader (Molecular Devi ces). 04. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). The growth. 0. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 5. 0 to 4. 0 1. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. 25–0. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). Data System. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. SpectraMAX 340. chen@case. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. Microplate Readers. Microplate Readers. Create custom fluorescence assays without having to purchase expensive filter sets. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. , Fremont, CA, USA). Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. 94 g calcium chloride dihydrate (both. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Manager: Yinghua Chen, PhD Contact Email: yinghua. edu Contact Phone: 216-368-8962. , Sunnyvale, CA) at 490 nm. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. The microtiter plate was then incubated in the dark for 2 h at 37°C. Includes: Computer Loaded with Softmax Pro 5. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. When necessary, samples were. Data were expressed as the mean percent viable cell vs. Fibrin clots were formed as described above with some modifications 46. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. If depot service is the best option for you we will assist you with logistics to help you keep. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 2. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. , Sunnyvale, CA). 306 plate air cal. SpectraMax® Microplate Readers and SoftMax® Pro Software. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 2 0. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. This reader only allows high performance HTRF measurement when assays are run in. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 2 were considered negative for IgM antibody. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Software (SoftMax Pro ® software or comparable plate reader software). Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. N-34-LB. #N207) LAL Reagent Water (Lonza cat. D. Acceptor filter: 665/10 nm. SpectraMAX 340. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Filterless readers provide consistent results with steady temperature regulation. One plate carrier adapter is provided with the instrument. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. Crowder, Ziqian Zeng, Alissa N. The absorbances were being measured at 515 nm. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. Unit powers. 190-1000 nm, tunable in 1 nm increments. No power cord or accessories included. Nitric Oxide Assay. The absorbances were transformed to velocity of substrate consumption (nmol/min). When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. TDP-43 stress granular cells were treated with 70% ice-cold. Immunofluorescence and Aggregation Analysis. , Sunnyvale, CA). The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. Wavelength Range. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. 303 unable to cal dark current. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. using SpectraMax i3 (Molecular devices, San Jose, USA). The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. MOLECULAR DEVICE M5. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. 6 g sodium acetate trihydrate and 2. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. , San Jose, CA) using Softmax Pro 7 software. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. 0200-0729. , Sunnyvale, CA). SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. A. Call 1-800-745-2710. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). 316 grating motor driver faulty. Percent Transmission Absorbance Competitor 0. Samples containing 25 μg of protein were separated on 12. Spectral Range. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). 81. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. After adding sample, the mixture was incubated for 30 min at 37°C, with one unit of enzymatic activity being defined as an increase in absorbance of 0. Wavelength(s) 340 to 850 nm; Add to Compare List. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Stat Fax 3200 Microplate Reader. TGFα release was calculated as described in the Results. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 0200-7027. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Awareness Technology, Inc. The concentration of TNFα in the experimental. 1 0 100 80 60 40 20 0 4. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. #ABS PLUS) Methods. Hydrolysis of compounds 9-15 was monitoredAbsorption was measured at 405 nm against 490 nm using a microplate reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). ScanLater Western Blot Detection Cartridge. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. Example: [Drawer]. Donor: 616/10 nm. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. 30. These isotypes cause antigen cross-linking by multimeric binding and self. 20. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. Briefly, cells were seeded into a. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. Each experiment was performed in duplicate and repeated at. SpectraMax Microplate Readers. One plate carrier adapter is provided with the instrument. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Awareness Technology, Inc. Cell culture 3T3 fibroblasts and RAW 264. SpectraMax Microplate Readers. used. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. SpectraMax Multi-Mode Microplate Readers. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. The SpectraMax® iD3 Multi Mode Microplate . Buffer (100 mmol L –1acetate buffer, pH 5. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). The VersaMax and SpectraMax 190 read 96-well microplates. 1. Other technologies such as imaging. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. used in the SPECTRAmax PLUS. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Call 1-800-745-2710. . #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. The reaction was stopped with 1 M hydrochloric acid after 15 min. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. 2. View the Molecular Devices website. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 05 ng/mL; progesterone: 2. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. 5. 312 gain calibration failed. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Setup recommendations for SpectraMax. Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Vessel Sprout Formation Assay. 1. Quick View. SpectraMax Multi-Mode Microplate Readers. SpectraMax Multi-Mode Microplate Readers. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The cell proliferation on different Ti discs was assessed by measuring the absorbance at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Devices, Sunnyvale, CA). Polystyrene plates (384-well) were precoated overnight at 4°C with 2 μg/ml of murine, human, or chimeric RANKL. 00 USD. Briefly, cells were seeded into a. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. 314 low lamp level warning. Upgradeable modules are also available including western blot, cell imaging, and fast. SpectraMax Multi-Mode Microplate Readers. 1: SpectraMax Plus 384 components. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Awareness Technology Inc. Details. Analysis of ALA by Ehrlich’s Reagent Assay. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. 0200-6117. Service parts for these models are not being produced and are no longer in stock. Molecular Devices SPECTRAmax. Lactate Dehydrogenase (LDH) Release. Assay setup for a 384-well low-volume plate. Isolation of RNA and RT-PCR. Manager: Yinghua Chen, PhD Contact Email: yinghua. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. 001/min at 425 nm. FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). Bioz Stars score: 86/100, based on 1 PubMed citations. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. 4. Wells were washed three times. 2 g/L solution; dilute 1:10 in HBSS. 0% and ± 0. using SpectraMax i3 (Molecular devices, San Jose, USA). The absorbance was measured at 340 nm for 2 min at a 15 s time interval. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. SpectraMax 190 reads 96-well and strip-well plates. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. The LDH level in the lavage fluid was calculated based on the standard curve, nor-malized with protein concentration and expressed as ng/mg protein. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax 340 from Molecular Devices). 7. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. 1% N-1-naphtylethylenediamine dihydrochloride, 2. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). the SpectraMax iD3 is a fantastic plate reader for a. , Sunnyvale, CA, USA) at 595 nm. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. California Lightworks uses only the highest quality LEDs from Osram. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Details. 5 stars - 1602 reviewsThis assay typically develops in 1. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. 5 - 2. The first time you replace an OEM lamp you must use the N-34-MLA.